Plant Physiol. Illumina
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Plant Physiology 59:1072-1075 (1977)
© 1977 American Society of Plant Biologists

This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Web of Science (10)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Devilliers, O. T.
Right arrow Articles by Ashton, F. M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Devilliers, O. T.
Right arrow Articles by Ashton, F. M.
Agricola
Right arrow Articles by Devilliers, O. T.
Right arrow Articles by Ashton, F. M.
Articles

Metabolic Activity of Isolated Leaf Cells of Phaseolus vulgaris in Relation to Leaf Development

O. T. Devilliers1 and Floyd M. Ashton

a Department of Botany, University of California, Davis, California 95616

Mesophyll cells were isolated from primary leaves of 5- to 21-day Phaseolus vulgaris plants. The rate of photosynthesis and respiration, and RNA, protein, and lipid synthesis was determined for these cells. Appropriate 14C substrates and product purification procedures were used for each process prior to liquid scintillation counting. The size of the leaves increased about 5-fold between days 5 and 11, and then remained relatively constant. The greatest increase in size occurred between days 5 and 6. The age of the leaf from which the cells were isolated had a pronounced effect on the rate of all of these processes. The largest changes occurred during the period of leaf expansion (days 5-11). Initially the rate of RNA, protein, and lipid synthesis increased rapidly, maintained a maximum rate for only 1 day (day 6 or day 7), and then declined. The rate of photosynthesis increased more slowly reaching a maximum at day 9, remained relatively constant until day 15, and then declined. The rate of respiration decreased during the first 4 days to low level which was maintained throughout the experiment. The time course patterns of these biochemical processes in isolated cells were similar to those which have been reported for intact leaves. It seems that isolation of leaf cells does not modify their metabolic activity.

1 Present address: Dept. of Biochemistry, University of Stellenbosch, Stellenbosch, South Africa.






HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
ASPB Publications PLANT PHYSIOLOGY® THE PLANT CELL
Copyright © 1977 by the American Society of Plant Biologists