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Proof for the Production of Cutinase by Fusarium solani f. pisi during Penetration into Its Host, Pisum sativum¹

^a Department of Agricultural Chemistry, Washington State University, Pullman, Washington 99164

Rabbit antibody to cutinase-I, isolated from Fusarium solani f. pisi, was conjugated to ferritin. With this ferritin-conjugated antibody it was shown that germinating spores of this fungus excreted cutinase during the penetration of the host pisum sativum. This result constitutes the most specific and strongest evidence for an enzymic penetration of a plant cuticle by a pathogen during infection.

¹ This work was supported in part by National Science Foundation Grant BMS 74-09351 and a grant from Washington State Tree Fruit Commission. Scientific Paper No. 4780, Project 2001, Agricultural Research Center, College of Agriculture, Washington State University, Pullman, Wash. 99164.

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