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Plant Physiology 60:40-43 (1977)
© 1977 American Society of Plant Biologists

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Articles

Effect of Glyphosate on Carrot and Tobacco Cells 1

Lloyd C. Haderlie2, Jack M. Widholm and Fred W. Slife

a Department of Agronomy, University of Illinois, Urbana, Illinois 61801

The growth of suspension-cultured carrot (Daucus carota L.) and tobacco (Nicotiana tabacum L. cv. Xanthi) cells was inhibited by glyphosate (N-[phosphonomethyl]glycine). This inhibition was reversed by adding combinations of phenylalanine, tyrosine, and tryptophan or casein hydrolysate. Casein hydrolysate and phenylalanine + tyrosine + tryptophan were the most effective treatments. Reversal of glyphosate-induced inhibition occurred only if the aromatic amino acids were added during the first 8 days of glyphosate incubation. Glyphosate uptake was not reduced when the aromatic amino acids or casein hydrolysate were added.

Even though phenylalanine biosynthesis is a suggested site for glyphosate action, inhibitory levels of glyphosate did not lower free phenylalanine concentrations in carrot cells within 10 days. 14C-Phenylalanine studies indicated that the metabolic pool size was, likewise, not decreased.

In carrot cells total free amino acids increased within 6 hours after glyphosate addition. Cell protein levels declined within 48 hours following glyphosate treatment.

Studies on 14C-thymidine and 14C-uridine incorporation were complicated by rapid metabolism of these compounds to 14CO2.

2 Present address: Dept. of Agronomy, University of Nebraska, Lincoln, Neb. 68583.

1 This work was supported by funds from the Illinois Agricultural Experiment Station. Research from Ph.D. thesis of senior author.






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Copyright © 1977 by the American Society of Plant Biologists