Plant Physiol. Illumina
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Plant Physiology 60:179-181 (1977)
© 1977 American Society of Plant Biologists

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Articles

A Rapid, Simple Method for Nuclei Isolation from Plant Protoplasts 1

Kanji Ohyama, Lawrence E. Pelcher and Dave Horn

a National Research Council of Canada, Prairie Regional Laboratory, Saskatoon, Saskatchewan S7N 0W9

A rapid, simple method for nuclei isolation and purification from soybean (Glycine max L. Merr.) protoplasts is described. The isolated nuclei exhibited active amino acid incorporation and RNA synthesis, but DNA synthesis was not detectable. Analysis by CsCl density gradient centrifugation showed that DNA isolated from nuclei had a single band, while DNA isolated from protoplasts consisted of three bands comprised of nuclear DNA, mitochondrial DNA, and chloroplast DNA.

1 NRCC No. 15964.






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Copyright © 1977 by the American Society of Plant Biologists