Plant Physiol. Journal of Pharmacology and Experimental Therapeutics
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Plant Physiology 60:197-202 (1977)
© 1977 American Society of Plant Biologists

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Articles

Kinetin Incorporated into Tobacco Callus Ribosomal RNA and Transfer RNA Preparations 1

Norimoto Muraia, Barbara J. Tallera, Donald J. Armstronga,2 and Folke Skooga

Melvin A. Mickeb and Heinrich K. Schnoesb

a Institute of Plant Development, Birge Hall, University of Wisconsin, Madison, Wisconsin 53706, Department of Biochemistry, University of Wisconsin, Madison, Wisconsin 53706

Kinetin, N6-furfuryladenine, was incorporated into tobacco (nicotiana tabacum L., var. Wis. No. 38) callus RNA isolated from rapidly growing tissue cultured in the presence of N6-furfuryladenine-8-14C or unlabeled kinetin. Approximately 0.7% of the radioactivity in the labeled kinetin added to the medium was recovered as N6-furfuryladenosine (fr6A) in the rRNA and tRNA preparations from the tobacco callus. The rRNA contained over 90% of these fr6 A moieties. The extent of kinetin incorporation was four times greater than that observed for N6-benzyladenine. The radiochemical purity of the recovered fr6 A was confirmed by three successive chromatographic purifications on Sephadex columns (LH-20 eluted with 35% ethanol, G-10 eluted with 20% ethanol, and LH-20 eluted with water). A cytokinin-active ribonucleoside with elution volumes corresponding to fr6 A was isolated from the tobacco callus rRNA preparation. This compound was analyzed by gas-liquid chromatography and rigorously characterized as N6-furfuryladenosine by gas-liquid chromatography-mass spectrometry of the trimethylsilyl derivative.

2 Present address: Department of Botany and Plant Pathology, Oregon State University, Corvallis, Oregon 97331.

1 This work was supported in part by the National Science Foundation research Grant BMS72-02226A02 to Folke Skoog and by the Research Committee of the Graduate School with funds from the Wisconsin Alumni Research Foundation.






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Copyright © 1977 by the American Society of Plant Biologists