Plant Physiology 60:379-383 (1977)
© 1977 American Society of Plant Biologists
Articles
Enzymic Assay of 10–7 to 10–14 Moles of Sucrose in Plant Tissues 1
Michael G. K. Jones2,
William H. Outlaw, Jr.3 and
Oliver H. Lowry
a Department of Pharmacology, Washington University Medical School, St. Louis, Missouri 63110
Procedures are described for measuring sucrose in plant extracts or freeze-dried tissue in the range between 10–7 and 10–14 moles. The method is based on the destruction of pre-existing glucose and fructose, followed by the hydrolysis of sucrose and reduction of NADP+ by a series of coupled enzymic reactions. Depending on the sensitivity required, the NADPH is determined directly with a spectrophotometer or a fluorometer, or is amplified as much as 30,000 times before fluorometric assay. The procedures suggested for the macro level are simpler than current methods, and those suggested for microanalysis are several orders of magnitude more sensitive.
With this technique, single palisade parenchyma cells and single spongy parenchyma cells of Vicia faba leaflets were each found to contain about 2.2 pmoles of sucrose.
2 Present address: University of Cambridge, Department of Biochemistry, Tennis Court Road, Cambridge, England.
3 To whom all correspondence should be sent. Present address: Department of Biology, Washington University, St. Louis, Mo. 63130.
1 Supported by National Science Foundation Grant PCM 76-02060 and American Cancer Society Grant BC-4S and by a Fellowship from the Science Research Council (U.K.) to M. G. K. J.
This article has been cited by other articles:
|
|
|
|
 
J. R. Seebauer, G. W. Singletary, P. M. Krumpelman, M. L. Ruffo, and F. E. Below
Relationship of source and sink in determining kernel composition of maize
J. Exp. Bot.,
November 16, 2009;
(2009)
erp324v1.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
T. Ventriglia, M. L. Kuhn, M. T. Ruiz, M. Ribeiro-Pedro, F. Valverde, M. A. Ballicora, J. Preiss, and J. M. Romero
Two Arabidopsis ADP-Glucose Pyrophosphorylase Large Subunits (APL1 and APL2) Are Catalytic
Plant Physiology,
September 1, 2008;
148(1):
65 - 76.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
P. D.R. van Heerden, G. Kiddle, T. K. Pellny, P. W. Mokwala, A. Jordaan, A. J. Strauss, M. de Beer, U. Schluter, K. J. Kunert, and C. H. Foyer
Regulation of Respiration and the Oxygen Diffusion Barrier in Soybean Protect Symbiotic Nitrogen Fixation from Chilling-Induced Inhibition and Shoots from Premature Senescence
Plant Physiology,
September 1, 2008;
148(1):
316 - 327.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. J. McCormick, M. D. Cramer, and D. A. Watt
Changes in Photosynthetic Rates and Gene Expression of Leaves during a Source Sink Perturbation in Sugarcane
Ann. Bot.,
January 1, 2008;
101(1):
89 - 102.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
E. A. Ainsworth, A. Rogers, A. D. B. Leakey, L. E. Heady, Y. Gibon, M. Stitt, and U. Schurr
Does elevated atmospheric [CO2] alter diurnal C uptake and the balance of C and N metabolites in growing and fully expanded soybean leaves?
J. Exp. Bot.,
February 1, 2007;
58(3):
579 - 591.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
H. Maeda, W. Song, T. L. Sage, and D. DellaPenna
Tocopherols Play a Crucial Role in Low-Temperature Adaptation and Phloem Loading in Arabidopsis
PLANT CELL,
October 1, 2006;
18(10):
2710 - 2732.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
E. A. Ainsworth, A. Rogers, L. O. Vodkin, A. Walter, and U. Schurr
The Effects of Elevated CO2 Concentration on Soybean Gene Expression. An Analysis of Growing and Mature Leaves
Plant Physiology,
September 1, 2006;
142(1):
135 - 147.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. M. Christ, E. A. Ainsworth, R. Nelson, U. Schurr, and A. Walter
Anticipated yield loss in field-grown soybean under elevated ozone can be avoided at the expense of leaf growth during early reproductive growth stages in favourable environmental conditions
J. Exp. Bot.,
July 1, 2006;
57(10):
2267 - 2275.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. Tamoi, M. Nagaoka, Y. Miyagawa, and S. Shigeoka
Contribution of Fructose-1,6-bisphosphatase and Sedoheptulose-1,7-bisphosphatase to the Photosynthetic Rate and Carbon Flow in the Calvin Cycle in Transgenic Plants
Plant Cell Physiol.,
March 1, 2006;
47(3):
380 - 390.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
F. Famiani, N. G. M. Cultrera, A. Battistelli, V. Casulli, P. Proietti, A. Standardi, Z.-H. Chen, R. C. Leegood, and R. P. Walker
Phosphoenolpyruvate carboxykinase and its potential role in the catabolism of organic acids in the flesh of soft fruit during ripening
J. Exp. Bot.,
November 1, 2005;
56(421):
2959 - 2969.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
H. Schluepmann, A. van Dijken, M. Aghdasi, B. Wobbes, M. Paul, and S. Smeekens
Trehalose Mediated Growth Inhibition of Arabidopsis Seedlings Is Due to Trehalose-6-Phosphate Accumulation
Plant Physiology,
June 1, 2004;
135(2):
879 - 890.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. Leterrier, R. Atanassova, L. Laquitaine, C. Gaillard, P. Coutos-Thevenot, and S. Delrot
Expression of a putative grapevine hexose transporter in tobacco alters morphogenesis and assimilate partitioning
J. Exp. Bot.,
April 1, 2003;
54(385):
1193 - 1204.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
R. Atanassova, M. Leterrier, C. Gaillard, A. Agasse, E. Sagot, P. Coutos-Thevenot, and S. Delrot
Sugar-Regulated Expression of a Putative Hexose Transport Gene in Grape
Plant Physiology,
January 1, 2003;
131(1):
326 - 334.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. C. Servaites and D. R. Geiger
Kinetic characteristics of chloroplast glucose transport
J. Exp. Bot.,
July 1, 2002;
53(374):
1581 - 1591.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
R. J. Laby, D. Kim, and S. I. Gibson
The ram1 Mutant of Arabidopsis Exhibits Severely Decreased beta -Amylase Activity
Plant Physiology,
December 1, 2001;
127(4):
1798 - 1807.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
W. H. Outlaw Jr. and S. Zhang
Single-cell dissection and microdroplet chemistry
J. Exp. Bot.,
April 1, 2001;
52(356):
605 - 614.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
U. Schurr, U. Heckenberger, K. Herdel, A. Walter, and R. Feil
Leaf development in Ricinus communis during drought stress: dynamics of growth processes, of cellular structure and of sink-source transition
J. Exp. Bot.,
September 1, 2000;
51(350):
1515 - 1529.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. J. Trevanion
Photosynthetic carbohydrate metabolism in wheat (Triticum aestivum L.) leaves: optimization of methods for determination of fructose 2,6-bisphosphate
J. Exp. Bot.,
June 1, 2000;
51(347):
1037 - 1045.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
D. B. Fisher and C. E. Cash-Clark
Gradients in Water Potential and Turgor Pressure along the Translocation Pathway during Grain Filling in Normally Watered and Water-Stressed Wheat Plants
Plant Physiology,
May 1, 2000;
123(1):
139 - 148.
[Abstract]
[Full Text]
|
|
|
|
|
|
|
F. Famiani, R. P. Walker, L. Tecsi, Z.-H. Chen, P. Proietti, and R. C. Leegood
An immunohistochemical study of the compartmentation of metabolism during the development of grape (Vitis vinifera L.) berries
J. Exp. Bot.,
April 1, 2000;
51(345):
675 - 683.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. Mérida, J. M. Rodríguez-Galán, C. Vincent, and J. M. Romero
Expression of the Granule-Bound Starch Synthase I (Waxy) Gene from Snapdragon Is Developmentally and Circadian Clock Regulated
Plant Physiology,
June 1, 1999;
120(2):
401 - 410.
[Abstract]
[Full Text]
|
|
|
|
|
|
|
F. M. Banks, S. P. Driscoll, M. A.J. Parry, D. W. Lawlor, J. S. Knight, J. C. Gray, and M. J. Paul
Decrease in Phosphoribulokinase Activity by Antisense RNA in Transgenic Tobacco. Relationship between Photosynthesis, Growth, and Allocation at Different Nitrogen Levels
Plant Physiology,
March 1, 1999;
119(3):
1125 - 1136.
[Abstract]
[Full Text]
|
|
|
|
|
|
|
J. Feng, R. J. Volk, and W. A. Jackson
Source and Magnitude of Ammonium Generation in Maize Roots
Plant Physiology,
November 1, 1998;
118(3):
835 - 841.
[Abstract]
[Full Text]
|
|
|
|
|
