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Articles

Responses of Enzymically Isolated Aleurone Cells of Oat to Gibberellin A₃¹

^a Department of Plant Pathology, Macdonald College of McGill University, Saint Anne de Bellevue, Province Quebec, Canada

Oat (Avena sativa L.) aleurone layer cells (spheroplasts) were isolated by maceration of the aleurone layer with a mixture of commercially available cellulase and pectinase. About 20% of the cells present in intact layers were released as spheroplasts and 79 ± 9% of the spheroplast population was viable as judged by methylene blue staining. The spheroplasts became disorganized in solutions containing less than 0.4 MD-mannitol. When the spheroplasts were incubated for 48 hours, total activities of acid p-nitrophenyl phosphatase and acid proteinase increased and protein levels decreased. These changes were not effected by gibberellin A₃. Isolated aleurone layers incubated under the same conditions as the spheroplasts showed reduced responses to gibberellin A₃. It is concluded that the necessary presence of an osmoticum limits the value of spheroplasts as a system for studying the mechanism of action of gibberellin A₃ in the aleurone cell.

¹ This research was supported by a NATO Postdoctoral Research Fellowship award.