Plant Physiol.
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Plant Physiology 60:606-608 (1977)
© 1977 American Society of Plant Biologists

This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Web of Science (159)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Knudson, L. L.
Right arrow Articles by Edwards, G. E.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Knudson, L. L.
Right arrow Articles by Edwards, G. E.
Agricola
Right arrow Articles by Knudson, L. L.
Right arrow Articles by Edwards, G. E.
Articles

Measurement of Ozone Injury by Determination of Leaf Chlorophyll Concentration 1

Linda L. Knudson, Theodore W. Tibbitts and Gerald E. Edwards

a Department of Horticulture, University of Wisconsin, Madison, Wisconsin 53706

A simple, rapid procedure is described for evaluating ozone injury to leaves of Phaseolus vulgaris L. cv. Pinto. Leaf chlorophyll is extracted with ethanol and analyzed spectrophotometrically; the concentration is expressed on the basis of leaf dry weight.

The per cent chlorophyll reduction of ozone-injured leaves was highly correlated with the per cent visible necrosis and chlorosis (r = 0.96). The variability in injury estimates with chlorophyll analysis was slightly less than with visual evaluation. An evaluation of chlorophyll a and b concentrations separately showed that the chlorophyll a/b ratio decreased with increasing amounts of injury. Chlorophyll determinations made for leaves harvested at intervals after an ozone treatment indicated that maximum chlorophyll reduction had occurred by 4 days.

This procedure for measuring ozone injury should be useful in eliminating the human bias associated with visible estimates of injury.

1 This research was financed in part by the College of Agricultural and Life Sciences, University of Wisconsin, Madison and in part with funds from the Wisconsin Power and Light Company, Madison Gas and Electric Company and Wisconsin Public Service Corporation and with Federal funds from the Environmental Protection Agency under Grant R803971. The contents do not necessarily reflect the views and policies of the Environmental Protection Agency or the power companies, nor does mention of trade names or commercial products constitute endorsement or recommendation for use.




This article has been cited by other articles:


Home page
 J. Environ. Qual.Home page
A. V. Di Vittorio
Pigment-based Identification of Ozone-Damaged Pine Needles as a Basis for Spectral Segregation of Needle Conditions
J. Environ. Qual., March 25, 2009; 38(3): 855 - 867.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
D. C. Gitz III, L. Liu-Gitz, J. W. McClure, and A. J. Huerta
Effects of a PAL inhibitor on phenolic accumulation and UV-B tolerance in Spirodela intermedia (Koch.)
J. Exp. Bot., April 1, 2004; 55(398): 919 - 927.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
ASPB Publications PLANT PHYSIOLOGY® THE PLANT CELL
Copyright © 1977 by the American Society of Plant Biologists