Plant Physiol.
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Plant Physiology 60:675-679 (1977)
© 1977 American Society of Plant Biologists

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Articles

Mitochondria of Isolated Plant Cells (Acer pseudoplatanus L.)

II. Copper Deficiency Effects on Cytochrome C Oxidase and Oxygen Uptake

Richard Bligny and Roland Douce

a D.R.F./Biologie Végétale, Centre d'Etudes Nucléaires et Université Scientifique et Médicale de Grenoble, 85 X 38041 Grenoble Cedex, France

The effects of copper deficiency on cell culture growth, cell respiration, mitochondrial oxidative properties, and electron transport chain have been studied with suspension-cultured sycamore cells (Acer pseudoplatanus L.). Within the range of the copper concentration studied (0.1-25 µg/1 of culture medium), the mean rate of cell division is independent of copper concentration. An initial copper concentration lower than 2 µg/1 limited the maximum density of population reached at the stationary phase of growth.

On a protein basis, the uncoupled O2 uptake rates were about the same for normal and copper-deficient cells. In contrast, the half-maximal inhibition of O2 uptake rate was obtained at greater KCN concentration in the normal cells (20 µM) compared to copper-deficient cells (2 µM). Similar results were obtained with the normal and copper-deficient sycamore cell mitochondria.

In the copper-deficient mitochondria, the concentration of the cytochrome aa3 was less than 0.02 nmol/mg mitochondrial protein or 1/20 of the normal rate. The b- and c-type cytochrome content was invariant with copper depletion. It appeared that cytochrome aa3 is present in large excess in normal cells. This work also indicated that cytochrome c is a very mobile molecule.






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Copyright © 1977 by the American Society of Plant Biologists