This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in Web of Science Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via CrossRef Citing Articles via Web of Science (20) Citing Articles via Google Scholar Google Scholar Articles by Singh, J. Articles by Siminovitch, D. Search for Related Content PubMed PubMed Citation Articles by Singh, J. Articles by Siminovitch, D. Agricola Articles by Singh, J. Articles by Siminovitch, D.

Articles

Relative Insensitivity of Mitochondria in Hardened and Nonhardened Rye Coleoptile Cells to Freezing in Situ¹

^a Research Branch, Agriculture Canada, Ottawa, Ontario K1A OC6, Canada

Mitochondria were isolated from excised coleoptiles of hardened and nonhardened winter rye (Secale cereale L. cv. Puma) seedlings which had been frozen extracellularly to different temperatures. No significant differences in the respiratory functions (ADP/O and respiratory control) were observed between mitochondria isolated from nonlethally and lethally frozen cells of both the hardened and nonhardened rye. These results suggest that mitochondria in situ can retain their normal function even after the cell was killed by the dehydrative stresses of extracellular freezing. Presumably, a different level of sensitivity to freezing stresses exists between mitochondrial and other membranous elements in the cell.

³ Contribution No. 504 of the Ottawa Research Station.

¹ This work was presented in abstract form at the 13th Annual Meeting of the Society for Cryobiology, August 1-6, 1976, Arlington, Virginia.