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Plant Physiology 60:771-774 (1977)
© 1977 American Society of Plant Biologists

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Isolation of Bacteria, Transforming Bacteria, and Bacteroids from Soybean Nodules 1

Te May Chinga and Saundra Hedtkea

William Newcombb

a Department of Agronomic Crop Science, Oregon State University, Corvallis, Oregon 97331, Department of Botany and Genetics, University of Guelph, Guelph, Ontario N1G 2W1, Canada

Postnuclei supernatant of soybean (Glycine max cv. Chippewa 64) nodule homogenate was fractionated by stepwise sucrose density gradient centrifugation into supernatant, endoplasmic reticulum and mitochondria, and three distinct bands with 1.22, 1.25, and 1.27 g/cm3 of peak density. Based on their enzymic activities, composition of electron transport components, and ultrastructural characteristics, the lightest band appears to be the mature bacteroids; the intermediate band the transforming bacteria; and the heaviest, the bacteria. The isolation procedure separates nodule symbionts into different functional and developmental fractions, and it may be a valuable tool for studies involving development, regulation, and senescence of bacteroids in the nodule.

1 This study was supported in part by a research operating grant from the National Research Council of Canada to R. C. P.

Oregon Agricultural Experiment Station Technical Paper No. 4498




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Copyright © 1977 by the American Society of Plant Biologists