Plant Physiol. Journal of Pharmacology and Experimental Therapeutics
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Plant Physiology 60:840-844 (1977)
© 1977 American Society of Plant Biologists

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Articles

Inhibition of Chloroplast Electron Transport Reactions by Trifluralin and Diallate 1

Steven J. Robinsona, Charles F. Yocuma and Hiroshi Ikumaa

Fumihiko Hayashib

a Department of Cell and Molecular Biology, Division of Biological Sciences, The University of Michigan, Ann Arbor, Michigan 48109, Criteria and Evaluation Division, Office of Pesticide Programs, United States Environmental Protection Agency, Washington, D.C. 20460

The herbicides trifluralin ({alpha},{alpha},{alpha}-trifluoro-2,6-dinitro-N, N-dipropyl-p-toluidine) and diallate (S-[2,3-dichloroallyl] diisopropylthiocarbamate) inhibit electron transport, ATP synthesis, and cytochrome f reduction by isolated spinach (Spinacia oleracea L.) chloroplasts. Both compounds inhibit noncyclic electron transport from H2O to ferricyanide more than 90% in coupled chloroplasts at concentrations less than 50 µM. Neither herbicide inhibits electron transport in assays utilizing only photosystem I activity, and the photosystem II reaction elicited by addition of oxidized p-phenylenediamine or 2,5-dimethylquinone is only partially inhibited by herbicide concentrations which block electron flow from H2O to ferricyanide. Inhibition of ATP synthesis parallels inhibition of electron flow in all noncyclic assay systems, and cyclic ATP synthesis catalyzed by either diaminodurene or phenazine metho-sulfate is susceptible to inhibition by both herbicides. These results indicate that trifluralin and diallate both inhibit electron flow in isolated chloroplasts at a point in the electron transport chain between the two photosystems.

1 Supported by EPA Research Contract No. 68-01-2482. Reference to herbicides in this document does not constitute an endorsement of their use by the EPA.






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Copyright © 1977 by the American Society of Plant Biologists