This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in Web of Science Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Web of Science (258) Citing Articles via Google Scholar Google Scholar Articles by Hüsken, D. Articles by Zimmermann, U. Search for Related Content PubMed PubMed Citation Articles by Hüsken, D. Articles by Zimmermann, U. Agricola Articles by Hüsken, D. Articles by Zimmermann, U.

Articles

Pressure Probe Technique for Measuring Water Relations of Cells in Higher Plants ¹

Institut für Biophysikalische Chemie (ICH/2) der Kernforschungsanlage Jülich, Postfach 1913, D-5170 Jülich, Germany

A new method is described for continuously measuring cell turgor pressure (P), hydraulic conductivity (L_p), and volumetric elastic modulus () in higher plant cells, using a pressure probe. This technique permits volume changes, V, and turgor pressure changes, P, to be determined with an accuracy of 10^–5 to 10^–6 µl and 3 to 5·10^–2 bar, respectively.

The main principle of the new method is the same as the pressure probe developed by Zimmermann and Steudle in which pressure is transmitted to a pressure transducer by means of an oil-filled capillary introduced into the cell. In order to use the pressure probe for small tissue cells, the effective compressible volume of the apparatus has to be sufficiently small in comparison to the volume of the cell itself. This is achieved by accurately fixing the oil/cell sap boundary in the very tip of the microcapillary by means of an electronic feedback mechanism, so that the effective volume of the apparatus is reduced to about 2 to 10% of the cell volume. In this way also, errors arising from compressibility of the apparatus and temperature fluctuations can be excluded.

Measurements on tissues cells of Capsicum annuum fruits yield values of 2 to 25 bar. Furthermore, can be shown to be a function of both cell turgor pressure and cell volume; increases with increasing turgor pressure and is higher in larger cells.

This article has been cited by other articles:

				H. Wada, M. A. Matthews, and K. A. Shackel Seasonal pattern of apoplastic solute accumulation and loss of cell turgor during ripening of Vitis vinifera fruit under field conditions J. Exp. Bot., April 1, 2009; 60(6): 1773 - 1781. [Abstract] [Full Text] [PDF]

				R. R. Lew and S. Nasserifar Transient responses during hyperosmotic shock in the filamentous fungus Neurospora crassa Microbiology, March 1, 2009; 155(3): 903 - 911. [Abstract] [Full Text] [PDF]

				A. Geitmann Experimental approaches used to quantify physical parameters at cellular and subcellular levels Am. J. Botany, October 1, 2006; 93(10): 1380 - 1390. [Abstract] [Full Text] [PDF]

				W. Fricke, G. Akhiyarova, W. Wei, E. Alexandersson, A. Miller, P. O. Kjellbom, A. Richardson, T. Wojciechowski, L. Schreiber, D. Veselov, et al. The short-term growth response to salt of the developing barley leaf J. Exp. Bot., March 1, 2006; 57(5): 1079 - 1095. [Abstract] [Full Text] [PDF]

				J. J. James, N. N. Alder, K. H. Muhling, A. E. Lauchli, K. A. Shackel, L. A. Donovan, and J. H. Richards High apoplastic solute concentrations in leaves alter water relations of the halophytic shrub, Sarcobatus vermiculatus J. Exp. Bot., January 1, 2006; 57(1): 139 - 147. [Abstract] [Full Text] [PDF]

				Y. Shimazaki, T. Ookawa, and T. Hirasawa The Root Tip and Accelerating Region Suppress Elongation of the Decelerating Region without any Effects on Cell Turgor in Primary Roots of Maize under Water Stress Plant Physiology, September 1, 2005; 139(1): 458 - 465. [Abstract] [Full Text] [PDF]

				D. A. Eisenbarth and A. R. Weig Dynamics of aquaporins and water relations during hypocotyl elongation in Ricinus communis L. seedlings J. Exp. Bot., July 1, 2005; 56(417): 1831 - 1842. [Abstract] [Full Text] [PDF]

				P. J. Franks Use of the pressure probe in studies of stomatal function J. Exp. Bot., June 1, 2003; 54(387): 1495 - 1504. [Abstract] [Full Text] [PDF]

				S. N. Shabala and R. R. Lew Turgor Regulation in Osmotically Stressed Arabidopsis Epidermal Root Cells. Direct Support for the Role of Inorganic Ion Uptake as Revealed by Concurrent Flux and Cell Turgor Measurements Plant Physiology, May 1, 2002; 129(1): 290 - 299. [Abstract] [Full Text] [PDF]

				N. Miyamoto, T. Ookawa, H. Takahashi, and T. Hirasawa Water Uptake and Hydraulic Properties of Elongating Cells in Hydrotropically Bending Roots of Pisum sativum L. Plant Cell Physiol., April 15, 2002; 43(4): 393 - 401. [Abstract] [Full Text] [PDF]

				A. D. Tomos and R. A. Sharrock Cell sampling and analysis (SiCSA): metabolites measured at single cell resolution J. Exp. Bot., April 1, 2001; 52(356): 623 - 630. [Abstract] [Full Text] [PDF]

				P. J. Franks, T. N. Buckley, J. C. Shope, and K. A. Mott Guard Cell Volume and Pressure Measured Concurrently by Confocal Microscopy and the Cell Pressure Probe Plant Physiology, April 1, 2001; 125(4): 1577 - 1584. [Abstract] [Full Text]

				L. J. Clark, W. R. Whalley, and P. B. Barraclough Partial mechanical impedance can increase the turgor of seedling pea roots J. Exp. Bot., January 1, 2001; 52(354): 167 - 171. [Abstract] [Full Text] [PDF]

				J. M. Wood Osmosensing by Bacteria: Signals and Membrane-Based Sensors Microbiol. Mol. Biol. Rev., March 1, 1999; 63(1): 230 - 262. [Abstract] [Full Text] [PDF]