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Plant Physiology 61:248-251 (1978)
© 1978 American Society of Plant Biologists

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Articles

Biosynthesis of Dolichyl Phosphate

Characterization and Site of Synthesis in Algae 1

H. Esteban Hopp, Gustavo R. Daleo2, Pedro A. Romero3 and Rafael Pont Lezica4

Departamento de Biología, Fundación Bariloche, C.C. 138, 8400 San Carlos de Bariloche, R.N., Argentina

This is the first report not only on the presence of polyprenyl phosphates and their site of synthesis in algae, but also on the formation of their sugar derivatives in this system.

A glucose acceptor lipid was isolated from the nonphotosynthetic alga Prototheca zopfii. The lipid was acidic and resistant to mild acid and alkaline treatments. The glucosylated lipid was labile to mild acid hydrolysis and resistant to phenol treatment and catalytic hydrogenation, as dolichyl phosphate glucose is. These results are consistent with the properties of an {alpha}-saturated polyprenyl phosphate.

The polyprenylic nature of the lipid was confirmed by biosynthesis from radioactive mevalonate. The [14C]lipid had the same chromatographic properties as dolichyl phosphate in DEAE-cellulose and Sephadex LH-20. Strong alkaline treatment and enzymic hydrolysis liberated free alcohols with chain lengths ranging from C90 to C105, C95 and C100 being the most abundant molecular forms. The glucose acceptor activity of the biosynthesized polyprenyl phosphate was confirmed.

The ability of different subcellular fractions to synthesize dolichyl phosphate was studied. Mitochondria and the Golgi apparatus were the sites of dolichyl phosphate synthesis from mevalonate.

2 Present address: Department of Biochemistry, Michigan State University, East Lansing, Michigan 48824.

3 Present address: Instituto de Investigaciones Bioquímicas, Fundación Campomar, Obligado 2490, 1428 Buenos Aires, Argentina, while on leave from the Universidad Católica de Valparaíso, Chile.

4 Career Investigator of the CONICET.

1 This work was partially supported by a grant from the CONICET (Consejo Nacional de Investigaciones Científicas y Técnicas, Argentina).






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Copyright © 1978 by the American Society of Plant Biologists