Plant Physiol.
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Plant Physiology 61:254-258 (1978)
© 1978 American Society of Plant Biologists

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Indole-3-acetic Acid Levels of Plant Tissue as Determined by a New High Performance Liquid Chromatographic Method 1

Philip B. Sweetser and Dennis G. Swartzfager

E. I. du Pont de Nemours and Company, Central Research and Development Department, Experimental Station, Wilmington, Delaware 19898

A method for the analysis of indole-3-acetic acid (IAA) in plant extracts has been developed based on high performance liquid chromatography separation of IAA on a microparticulate strong anion exchange column followed by quantitation with two selective detectors: an electrochemical, carbon paste amperometric detector and/or a fluorescence detector. The detection limit for IAA is less than 1 nanogram with the fluorescence detector and less than 50 picograms with the electrochemical detector.

The IAA levels are reported for various tissues of wheat, pinto beans, soybeans, cotton, and corn.

1 Central Research and Development Department Contribution No. 2493.




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[Abstract] [Full Text] [PDF]


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Copyright © 1978 by the American Society of Plant Biologists