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Plant Physiology 61:318-322 (1978)
© 1978 American Society of Plant Biologists

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Articles

Distribution of Incorporated, Synthetic Cytokinins in Ribosomal RNA Preparations from Tobacco Callus 1

Norimoto Murai, Donald J. Armstrong2, Barbara J. Taller and Folke Skoog

Institute of Plant Development, Birge Hall, University of Wisconsin, Madison, Wisconsin 53706

The distribution of incorporated synthetic cytokinins (N6-[8-14C]benzyladenine ([8-14C]bzl6Ade) and N6[8-14C]furfuryladenine ([8-14C]fr6Ade) in ribosomal RNA prepared from tobacco callus (Nicotiana tabacum L. var. Wis. No. 38) grown in the presence of one of these for 25 or 26 days has been studied. The rRNA of tissue supplied with [8-14C]bzl6Ade or [8-14C]fr6Ade was fractionated by methylated albumin-Kieselguhr column chromatography and preparative gel electrophoresis, respectively. In each case about 80% of the incorporated cytokinin was recovered as the ribonucleoside [8-14C]bzl6A or [8-14C]fr6A in the rRNA peak after the fractionations. [8-14C]fr6A was found associated with both the 18S and 25S rRNA components in quantities roughly proportional to their 260 nm absorbance. This pattern of apparently nonspecific association was not affected by prior denaturation of the RNA with formamide.

The distribution of [8-14C]fr6A moieties in mono- and oligonucleotides derived from combined treatment of [8-14C]fr6Ade-labeled rRNA with ribonuclease T1 and pancreatic ribonuclease A was measured by fractionating the digest on a DEAE-cellulose column in the presence of 7 M urea and determining the [8-14C]fr6A content in each fraction. The [8-14C]fr6A content in the oligonucleotides varied from 46 to 210 µmol/mol of adenosine (A). The mol ratio of [8-14C]fr6A to A was three to four times greater for oligonucleotides containing uridine or cytidine ([A]nUp or [A]nCp) than for those containing quanosine ([A]nGp).

2 Present address: Department of Botany and Plant Pathology, Oregon State University, Corvallis, Oregon 97331.

1 This work was supported in part by National Science Foundation Research Grant BMS72-02226A02 to F. S.






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Copyright © 1978 by the American Society of Plant Biologists