Plant Physiol.
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Plant Physiology 61:339-341 (1978)
© 1978 American Society of Plant Biologists

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Articles

Development and Properties of a Wax Ester Hydrolase in the Cotyledons of Jojoba Seedlings 1

Anthony H. C. Huang, Robert A. Moreau2 and Kitty D. F. Liu

Department of Biology, University of South Carolina, Columbia, South Carolina 29208

The activity of a wax ester hydrolase in the cotyledons of jojoba (Simmondsia chinensis) seedlings increased drastically during germination, parallel to the development of the gluconeogenic process. The enzyme at its peak of development was obtained in association with the wax body membrane, and its properties were studied. It had an optimal activity at alkaline pH (8.5-9). The apparent Km value for N-methylindoxylmyristate was 93 µM. It was stable at 40 C for 30 min but was inactivated at higher temperature. Various divalent cations and ethylenediaminetetraacetate had little effect on the activity. p-Chloromercuribenzoate was a strong inhibitor of the enzyme activity, and its effect was reversed by subsequent addition of dithiothreitol. It had a broad substrate specificity with highest activities on monoglycerides, wax esters, and the native substrate (jojoba wax).

2 Recipient of a W. Gordon Belser graduate fellowship.

1 This work was supported by National Science Foundation Grant BMS 75-02320.






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Copyright © 1978 by the American Society of Plant Biologists