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Plant Physiology 61:354-356 (1978)
© 1978 American Society of Plant Biologists

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Articles

Partial Characterization of Steryl Ester Biosynthesis in Spinach Leaves

Raymond E. Garcia1 and J. Brian Mudd

Department of Biochemistry and Statewide Air Pollution Research Center, University of California, Riverside, California 92521

Acetone powders of a 20,000g pellet fraction from spinach leaves (Spinacia oleracea L.) synthesized [4-14C]cholesteryl esters when incubated with [4-14C]cholesterol. The reaction was inhibited by digitonin. There was a reciprocal relationship between the decline of label in cholesterol and its incorporation into cholesteryl ester, indicating that free cholesterol was the direct precursor for cholesteryl ester biosynthesis. The hydrolysis of cholesteryl [1-14C]palmitate into free cholesterol and [1-14C]palmitate was not detected in these acetone powder preparations. Exogenous cholesteryl palmitate had no effect on the esterification of [4-14C]cholesterol. The data indicate that an esterase-type mechanism was not involved in the biosynthesis of these steryl esters. Label from [1-14C]palmitoyl-CoA was incorporated into steryl esters when incubated with spinach leaf acetone powder preparations. The optimal buffer for steryl ester biosynthesis was 2-(N-morpholino)ethanesulfonate and the optimal pH was 6. Iodoacetamide, N-ethylmaleimide, and dithiothreitol had no effect on the esterification reaction. Ethylenediaminetetraacetate, MgCl2, CaCl2, MnCl2, and ZnSO4 inhibited at concentrations of 10 to 30 mM.

1 Supported by a predoctoral National Science Foundation Traineeship. Present address: Biomedical Sciences Division, Lawrence Livermore Laboratory, University of California, Livermore, California 94550.




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Q. Chen, L. Steinhauer, J. Hammerlindl, W. Keller, and J. Zou
Biosynthesis of Phytosterol Esters: Identification of a Sterol O-Acyltransferase in Arabidopsis
Plant Physiology, November 1, 2007; 145(3): 974 - 984.
[Abstract] [Full Text] [PDF]


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Copyright © 1978 by the American Society of Plant Biologists