Plant Physiol.
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Plant Physiology 61:442-446 (1978)
© 1978 American Society of Plant Biologists

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Articles

Properties of Pea Seedling Uracil Phosphoribosyltransferase and Its Distribution in Other Plants 1

Ray A. Bressan2, Michael G. Murray3, James M. Gale4 and Cleon W. Ross

Department of Botany and Plant Pathology, Colorado State University, Fort Collins, Colorado 80523

A uracil phosphoribosyltransferase (UMP-pyrophosphorylase) was found in several angiosperms and was partially purified from epicotyls of pea (Pisum sativum L. cv. Alaska) seedlings. Its pH optimum was about 8.5; its required approximately 0.3 mM MgCl2 for maximum activity but was inhibited by MnCl2; its molecular weight determined by chromatography on Sephadex G-150 columns was approximately 100,000; its Km values for uracil and 5-phosphorylribose 1-pyrophosphate were 0.7 µM and 11 µM; and it was partially resolved from a similar phosphoribosyltransferase converting orotic acid to orotodine 5'-phosphate. Enzyme fractions containing both uracil phosphoribosyl transferase and orotate phosphoribosyltransferase converted 6-azauracil and 5-fluorouracil to products with chromatographic properties of 6-azauradine 5'-phosphate and 5-fluorouridine 5'-phosphate. Uracil phosphoribosyltransferase probably functions in salvage of uracil for synthesis of pyrimidine nucleotides.

2 Present address: Michigan State University, ERDA Plant Research Laboratory, East Lansing, Mich. 48824.

3 Present address: Department of Plant Biology, Carnegie Institution of Washington, Stanford, Calif. 94305.

4 Present address: Department of Botany, University of Georgia, Athens, Ga. 30602.

1 This research was supported by Grant GB 36480 from the National Science Foundation.






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Copyright © 1978 by the American Society of Plant Biologists