Plant Physiol.
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Plant Physiology 61:460-464 (1978)
© 1978 American Society of Plant Biologists

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Articles

Physicochemical Characterization of Mitochondrial DNA from Soybean

Richard M. Synenki, Charles S. Levings, III and Dilip M. Shah1

Max-Planck-Institut für Molekulare Genetik, Ihnestrasse 63-73, 1000 Berlin 33 (Dahlem), Germany

Mitochondrial DNA (mtDNA) of soybean (Glycine max L.) was isolated and its buoyant density was contrasted with that of nuclear (nDNA) and chloroplast (ctDNA) DNA. Each of the three DNAs banded at a single, characteristic buoyant density when centrifuged to equilibrium in a CsCl gradient. Buoyant densities were 1.694 g/cm3 for nDNA and 1.706 g/cm3 for mtDNA. These values correspond to G-C contents of 34.7 and 46.9%, respectively. Covalently closed, circular mtDNA molecules were isolated from soybean hypocotyls by ethidium bromide-cesium chloride density gradient centrifugation. Considerable variation in mtDNA circle size was observed by electron microscopy. There were seven apparent size classes with mean lengths of 5.9 µm (class 1), 10 µm (class 2), 12.9 µm (class 3), 16.6 µm (class 4), 20.4 µm (class 5), 24.5 µm (class 6), and 29.9 µm (class 7). In addition, minicircles were observed in all preparations. Partially denatured, circular mtDNA molecules with at least one representative from six of the seven observed size classes were mapped. In class 4, there appear to be at least three distinct denaturation patterns, indicating heterogeneity within this class. It is proposed that the mitochondrial genome of soybean is distributed among the different size circular molecules, several copies of the genome are contained within these classes and that the majority of the various size molecules may be a result of recombination events between circular molecules.

1 Present address: Population Genetics Section, Environmental Mutagenesis Branch, National Institute for Environmental Health Sciences, P.O. Box 12233, Research Triangle Park, North Carolina 27709.






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Copyright © 1978 by the American Society of Plant Biologists