This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Siefermann-Harms, D. Articles by Douce, R. Search for Related Content PubMed PubMed Citation Articles by Siefermann-Harms, D. Articles by Douce, R. Agricola Articles by Siefermann-Harms, D. Articles by Douce, R.

Articles

Light-induced Changes of the Carotenoid Levels in Chloroplast Envelopes ¹

Jacques Joyard and Roland Douce

Institut für Biologie I, Abt. Allgemeine Botanik und Pflanzenphysiologie, Universität Tübingen, D-7400 Tübingen, German Federal Republic, Centre d'Études Nucléaires and Université Scientifique et Medicale de Grenoble, Biologie Végétale, 85 x 38041 Grenoble Cedex, France

The carotenoid content of thylakoids and envelopes isolated from dark-or light-treated spinach (Spinacia oleracea L.) chloroplasts was compared. In thylakoids, light induced a decrease of violaxanthin parallel with a stoichiometric increase of zeaxanthin due to violaxanthin deepoxidation. In envelopes, violaxanthin was also decreased and the relative decrease was similar to thylakoids, but zeaxanthin increase was small resulting in an over-all decrease of the amount of envelope carotenoids. When violaxanthin deepoxidation in thylakoids was partly inhibited by 10 nM nigericin, violaxanthin decrease in the envelope was inhibited to a similar degree.

These observations together with the absence of deepoxidase activity in isolated envelopes plus added stroma proteins suggest that light-induced violaxanthin decrease in the envelope is not caused by an envelope or stroma deepoxidase but results from violaxanthin exchange between envelope and thylakoids.

This article has been cited by other articles:

				J. Joyard, M. Ferro, C. Masselon, D. Seigneurin-Berny, D. Salvi, J. Garin, and N. Rolland Chloroplast Proteomics and the Compartmentation of Plastidial Isoprenoid Biosynthetic Pathways Mol Plant, November 12, 2009; (2009) ssp088v1. [Abstract] [Full Text] [PDF]

				F. Li, R. Vallabhaneni, J. Yu, T. Rocheford, and E. T. Wurtzel The Maize Phytoene Synthase Gene Family: Overlapping Roles for Carotenogenesis in Endosperm, Photomorphogenesis, and Thermal Stress Tolerance Plant Physiology, July 1, 2008; 147(3): 1334 - 1346. [Abstract] [Full Text] [PDF]

				A. D. Hieber, O. Kawabata, and H. Y. Yamamoto Significance of the Lipid Phase in the Dynamics and Functions of the Xanthophyll Cycle as Revealed by PsbS Overexpression in Tobacco and In-vitro De-epoxidation in Monogalactosyldiacylglycerol Micelles Plant Cell Physiol., January 15, 2004; 45(1): 92 - 102. [Abstract] [Full Text] [PDF]

				J. T. Chernys and J. A.D. Zeevaart Characterization of the 9-Cis-Epoxycarotenoid Dioxygenase Gene Family and the Regulation of Abscisic Acid Biosynthesis in Avocado Plant Physiology, September 1, 2000; 124(1): 343 - 354. [Abstract] [Full Text]