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Plant Physiology 61:544-548 (1978) © 1978 American Society of Plant Biologists Characteristics of a Galactose-adapted Sugarcane Cell Line Grown in Suspension Culture 1Department of Crop Sciences, Experiment Station, Hawaiian Sugar Planters' Association, Aiea, Hawaii 96701
Although D-galactose is normally toxic to sugarcane (Saccharum sp.) cells, a cell line that grows on 100 mM galactose has been propagated. Nonadapted cells in a medium containing galactose instead of sucrose accumulate UDP-galactose; these cells also have much lower UDP-galactose 4-epimerase (EC 5.1.3.2) activity than do adapted cells. This enzyme may determine whether or not galactose will cause toxicity symptoms to develop. The growth rate of galactose-adapted cells is similar to most cell lines on several other carbohydrates. The galactose-adapted cells are also similar to sucrose stock cells in cell wall composition and sugar phosphate concentrations, but, like the nonadapted cells, accumulate free galactose.
1 This investigation was supported in part with funds provided by the United States Department of Agriculture Agreement No. 12-14-5001-29. Published with the approval of the Director as Paper No. 417 in the Journal Series of the Experiment Station, Hawaiian Sugar Planters' Association.
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