Plant Physiol. Drug Metab Dispos
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Plant Physiology 61:779-784 (1978)
© 1978 American Society of Plant Biologists

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Articles

Role of Lectins in Plant-Microorganism Interactions

II. Distribution of Soybean Lectin in Tissues of Glycine max (L.) Merr. 1

Steven G. Pueppke2 and Wolfgang D. Bauer3

Kenneth Keegstra4 and Ardene L. Ferguson

Charles F. Kettering Research Laboratory, 150 East South College Street, Yellow Springs, Ohio 45387, Department of Microbiology, State University of New York, Stony Brook, New York 11790

Three different assay procedures have been used to quantitate the levels of soybean (Glycine max [L.] Merr.) lectin in various tissues of soybean plants. The assays used were a standard hemagglutination assay, a radioimmunoassay, and an isotope dilution assay. Most of the lectin in seeds was found in the cotyledons, but lectin was also detected in the embryo axis and the seed coat. Soybean lectin was present in all of the tissues of young seedlings, but decreased as the plants matured and was not detectable in plants older than 2 to 3 weeks. Soybean lectin isolated from seeds of several soybean varieties were identical when compared by several methods.

2 Present address: Department of Biology, University of Missouri, St. Louis, 8001 Natural Bridge Road, St. Louis, Missouri 63121.

3 To whom reprint requests should be sent.

4 Present address: Department of Botany, University of Wisconsin, Madison, Wisconsin 53706.

1 Supported in part by National Science Foundation Grants BMS 75-17710 and BMS 76-00913.






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Copyright © 1978 by the American Society of Plant Biologists