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Effect of Triacontanol on Plant Cell Cultures in Vitro ¹

Peter Carlson

Department of Horticulture, Pesticide Research Center, Michigan State University, East Lansing, Michigan 48824, Department of Crop and Soil Sciences, Michigan State University, East Lansing, Michigan 48824

Triacontanol [CH₃(CH₂)₂₈CH₂OH] increased growth in vitro of cell cultures of haploid tobacco (Nicotiana tabacum). The fresh weight of cell cultures of tomato (Lycopersicon esculentum), potato (Solanum tuberosum), bean (Phaseolus vulgaris), and barley (Hordeum vulgare x H. jubatum) was also increased. The increase in growth of tobacco callus seems to have been due to an increase in cell number. Another long chain alcohol, octocosanol [CH₃(CH₂)₂₆CH₂OH], did not increase the growth of tobacco cell cultures.