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Plant Physiology 61:871-877 (1978)
© 1978 American Society of Plant Biologists

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Articles

Isoenzymes of the Glycolytic and Pentose Phosphate Pathways in Proplastids from the Developing Endosperm of Ricinis communis L. 1

P. David Simcox2 and David T. Dennis

Department of Biology, Queen's University, Kingston, Ontario Canada K7L 3N6

Two isoenzymes each of hexose-P isomerase, aldolase and 6-P-gluconate dehydrogenase have been found in the endosperm of developing castor beans (Ricinus communis L.). One isoenzyme for each activity is present in the proplastid fraction. Only one form of glucose-6-P dehydrogenase was found. It is suggested that the partition of an enzyme activity between cytosol and plastid is regulated by the synthesis of isoenzymes which are subcellular site specific. In addition, this report describes the use of diethylaminoethyl-Sephadex A-25 sievorptive chromatography for the preparation of plant enzymes.

2 Present address: Diamond Shamrock Corp., Painesville, Ohio.

1 Supported by Grant 5051 from the National Research Council of Canada.




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ASPB Publications PLANT PHYSIOLOGY® THE PLANT CELL
Copyright © 1978 by the American Society of Plant Biologists