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Plant Physiology 62:134-138 (1978)
© 1978 American Society of Plant Biologists

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Effects of Allopurinol [4-Hydroxypyrazolo(3,4-d)Pyrimidine] on the Metabolism of Allantoin in Soybean Plants

Shinsuke Fujihara and Masuro Yamaguchi

Department of Agricultural Chemistry, College of Agriculture, University of Osaka Prefecture, 4-cho, Mozuumemachi, Sakai, Osaka 591, Japan

Some studies on the effects of xanthine oxidase inhibitor allopurinol [4-hydroxypyrazolo(3,4-d)pyrimidine] on allantoin metabolism of soybean plants (Glycine max cv. Tamanishiki) are reported. Soybean seedlings, aseptically germinated for 96 hours on agar containing 1 millimolar allopurinol, contained only slight amounts of allantoin, allantoic acid, and urea as compared with controls. Analysis of purines and pyrimidines of the allopurinol-treated seedlings showed marked accumulation of xanthine both in the cotyledons and seedling axes. No hypoxanthine accumulation was found. Xanthine accumulation due to allopurinol treatment was relatively low after the cotyledons had fallen. For nodulated plants, allopurinol caused a significant drop in allantoin (+allantoic acid) in the stems and nodules, accompanied by a striking accumulation of xanthine in the nodules. The xanthine concentration in the nodules far exceeded that in the germinated seedlings. Allopurinol at a concentration of 50 micromolar strongly inhibited xanthine oxidase prepared from soybean nodules.

The results suggested that the main pathway of allantoin formation in soybean plants was through purine decomposition, via xanthine-uric acid. It was specially noted that a very active purine-decomposing system existed in soybean nodules.




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P. M.C. Smith and C. A. Atkins
Purine Biosynthesis. Big in Cell Division, Even Bigger in Nitrogen Assimilation
Plant Physiology, March 1, 2002; 128(3): 793 - 802.
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E. H. NEWCOMB and S. R. TANDON
Uninfected Cells of Soybean Root Nodules: Ultrastructure Suggests Key Role in Ureide Production
Science, June 19, 1981; 212(4501): 1394 - 1396.
[Abstract] [PDF]


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Copyright © 1978 by the American Society of Plant Biologists