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Availability of Chloride Affects the Balance between Potassium Chloride and Potassium Malate in Guard Cells of Vicia faba L. ¹

MSU-ERDA Plant Research Laboratory, Michigan State University, East Lansing, Michigan 48824

Electron probe microanalysis for K and Cl and enzymic determination of malate were performed on epidermal strips of Vicia faba L. which had been incubated with 0.1 equivalent of K⁺ per liter in the absence or presence of Cl^–. In the absence of Cl^–, iminodiacetate, a presumed impermeant zwitterion, served as anion. With no Cl^– in the medium, 91% of the K⁺ imported into the guard cells during stomatal opening was neutralized by malate production; import of Cl^– (presumably from the rest of the epidermal tissue) contributed 6%. In the presence of Cl^–, 50% of the necessary negative charges were provided by malate synthesis, 45% by Cl^– import. Stomatal opening was not obviously affected by the chloride concentration in the incubation medium, but malate production declined roughly linearly with the logarithm of [Cl^–] between 10^–5 and 10^–1 equivalent per liter.

¹ Research supported by the U.S. Energy Research and Development Administration under Contract EY-76-C-02-1338.

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