Plant Physiol. Drug Metab Dispos
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Plant Physiology 62:88-93 (1978)
© 1978 American Society of Plant Biologists

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Articles

Activity of Thylakoid-bound Ribosomes in Pea Chloroplasts 1

Ruth Alscher2, Richard Patterson3 and Andre T. Jagendorf4

Division of Biological Sciences, Cornell University, Ithaca, New York 14853

Pea (Pisum sativum) chloroplast thylakoid membranes were prepared by washing in hypotonic buffers. These membranes contained bound ribosomes which were active in protein synthesis when supplemented with soluble components from a strain of Escherichia coli low in ribonuclease. After dissolving the membranes by Triton and purification of the ribosomes, sucrose density gradient profiles indicated the presence of polysomal material as well as monomeric ribosomes. Most of the products of protein synthesis remained associated with the thylakoid membranes even after ribosomes were removed completely by high salt concentrations in the absence of Mg2+. Of the newly formed products, 50% could be digested by pronase, while the remainder were protected by their association with the thylakoid membranes. The products are likely to be a mixture of intrinsic and extrinsic membrane proteins, with only the former completely protected by the membranes from attack by proteases.

2 Recipient of National Institutes of Health Postdoctoral Fellowship 1 F32 GM05172. Current address: New York State Agricultural Experiment Station, Cornell University, Geneva, New York 14456.

3 Present address: Agricultural Division, CIBA-GEIGY Corp., P.O. Box 11422, Greensboro, North Carolina 27409.

4 To whom reprint requests should be sent.

1 Supported in part by National Science Foundation Grant BMS-74-13534.






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Copyright © 1978 by the American Society of Plant Biologists