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Enzymes of Krebs-Henseleit Cycle in Vitis vinifera L

II. Arginosuccinate Synthetase and Lyase ¹

Department of Viticulture and Enology, University of California, Davis, California 95616

Arginosuccinate (ASA) synthetase and lyase activities were detected in extracts from Vitis vinifera L. cv. Chenin blanc mature leaves and seedlings. Optimum reaction conditions for ASA synthetase were 10 millimolar L-citrulline, 7.5 millimolar L-aspartate, 3 to 4 millimolar ATP, 12 millimolar Mg²⁺ (pH 7.5 to 8.0), enzyme extract up to equivalent of about 200 milligrams of fresh tissue, and incubation temperature of 38 to 40 C. Optimum reaction conditions for ASA lyase were 4 millimolar ASA-K salt (pH 7.3 to 7.8), amount of extract up to equivalent of about 180 milligrams of fresh tissue, and incubation temperature of 38 to 40 C.

¹ This report is part of a dissertation submitted by K. A. R. in partial fulfillment of the requirements for the Ph.D. degree at the University of California, Davis.