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Plant Physiology 62:363-367 (1978)
© 1978 American Society of Plant Biologists

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Articles

Characterization of the Enzyme Responsible for Nopaline and Ornaline Synthesis in Sunflower Crown Gall Tissues 1

Dennis W. Sutton, John D. Kemp and Ethan Hack

Plant Disease Resistance Research Unit, Agricultural Research Service, United States Department of Agriculture, and Department of Plant Pathology, University of Wisconsin, Madison, Wisconsin 53706

Extracts prepared from sunflower (Helianthus annuus L.) crown gall tissues induced by Agrobacterium tumefaciens strains C58 and T37 (nopaline utilizers) catalyze the synthesis of nopaline and ornaline. These compounds are not synthesized in extracts of crown gall tissues induced by strains B6, 15955 (octopine utilizers), and AT1 (utilizes neither octopine nor nopaline) or in extracts of habituated sunflower callus. Both synthetic activities require NADPH, {alpha}-ketoglutarate, and either arginine or ornithine; histidine and lysine will not substitute. Incorporation of arginine or ornithine into product is inhibited by the other substrate but not by histidine or lysine. On the basis of inhibition and Km data, both activities appear to be catalyzed by one enzyme and the same enzyme is apparently present in crown gall tissues induced by strains C58 and T37.

1 Research cooperative with the College of Agricultural and Life Sciences, University of Wisconsin, Madison, and the Agricultural Research Service, United States Department of Agriculture. E. H. was the recipient of Public Health Service Predoctoral Training Grant ST32GM07215.




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A. Caplan, L. Herrera-Estrella, D. Inze, E. Van Haute, M. Van Montagu, J. Schell, and P. Zambryski
Introduction of Genetic Material into Plant Cells
Science, November 18, 1983; 222(4625): 815 - 821.
[Abstract] [PDF]


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Copyright © 1978 by the American Society of Plant Biologists