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Plant Physiology 62:781-783 (1978)
© 1978 American Society of Plant Biologists

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Depolarization of the Electrogenic Transmembrane Electropotential of Zea mays L. by Bipolaris (Helminthosporium) maydis Race T Toxin, Azide, Cyanide, Dodecyl Succinic Acid, or Cold Temperature 1

Stuart M. Mertz, Jr.2 and Charles J. Arntzen

United States Department of Agriculture, Science and Education Administration, Department of Botany, University of Illinois, Urbana, Illinois 61801

The transmembrane electrical potential of root cells of Zea mays L. cv. W64A in a modified 1x Higinbotham solution was partially depolarized by semipurified toxin obtained from Bipolaris (Helminthosporium) maydis race T. At a given toxin concentration depolarization of Texas cytoplasm cells was much greater than for normal cytoplasm cells. This observation correlated directly to the differential host susceptibility to the fungus. The time course and magnitude of depolarization were dependent on toxin concentration; at high concentration the electropotential difference change was rapid. Cortex cells depolarized more slowly than epidermal cells indicating that the toxin slowly permeated intercellular regions. Toxin concentrations which affected electropotential difference were of the same magnitude as those required to inhibit root growth, ion uptake, and mitochondrial processes.

Azide, cyanide, and cold temperature (5 C) gave the same partial depolarization as did the toxin. Dodecyl succinic acid caused complete depolarization. These and other data indicate that one of the primary actions of the toxin is to inhibit electrogenic ion pumps in the plasmalemma.

2 Present address: Monsanto Agricultural Products Co., 800 North Lindbergh Blvd., St. Louis, Missouri 63166.

Abbreviations: T or Tcms: Texas cytoplasm male-sterile; N: normal cytoplasm; PD: electropotential difference; DSA: dodecyl succinic acid; n: number of PD measurements averaged.

1 This research was supported in part by funds from the Illinois Agricultural Experiment Station.






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Copyright © 1978 by the American Society of Plant Biologists