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Plant Physiology 62:794-797 (1978)
© 1978 American Society of Plant Biologists

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Simultaneous Separation of Acidic and Basic Isoperoxidases in Wounded Potato Tissue by Acrylamide Gel Electrophoresis 1

Rolf Borchert

Charles J. Decedue

Department of Botany and of Physiology and Cell Biology, University of Kansas, Lawrence, Kansas 66045, Enzyme Laboratory, University of Kansas, Lawrence, Kansas 66045

Preparation and use of a newly developed pH 4.3 horizontal thin layer acrylamide gel which permits the simultaneous separation of acidic and basic isoperoxidases in up to 30 samples is described. Use of cytochrome c, horseradish peroxidase, and a purified potato isoperoxidase as internal standards for a range in isoelectric points of peroxidases from pH 3 to 11 is introduced to facilitate comparison of results obtained with different materials and different methods. Distribution of tissue-specific isoperoxidases in different cell layers of wounded potato (Solanum tuberosum L.) tissue is shown and their purification described. Evidence for the in vitro degradation of basic potato isoperoxidases resulting in more acidic forms similar to isoperoxidases occurring in wounded potato tissue is presented. The significance of this observation for the postulated differential function of different isoperoxidases is discussed.

1 Supported, in part, by National Science Foundation Grant BG 29313, and by a grant from the University of Kansas General Research Fund.




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M. A. Bernards, W. D. Fleming, D. B. Llewellyn, R. Priefer, X. Yang, A. Sabatino, and G. L. Plourde
Biochemical Characterization of the Suberization-Associated Anionic Peroxidase of Potato
Plant Physiology, September 1, 1999; 121(1): 135 - 146.
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Copyright © 1978 by the American Society of Plant Biologists