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Plant Physiology 63:14-19 (1979)
© 1979 American Society of Plant Biologists

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Articles

Quantitation of 1,4-Benzoxazin-3-ones in Maize by Gas-Liquid Chromatography 1

Michael D. Woodward2, Luis J. Corcuera3, John P. Helgeson4, Arthur Kelman and Christen D. Upper4

a Department of Plant Pathology, University of Wisconsin, Madison, Wisconsin, 53706

A gas-liquid chromatographic (GLC) procedure is reported for the quantitation of the trimethylsilyl (TMS) derivatives of substituted 2-hydroxy-2H-1,4-benzoxazin-3(4H)-ones (2-hydroxy-2H-1,4-benzoxazin-3(4H)-one[HBOA]; 2-hydroxy-7-methoxy-2H-1,4-benzoxazin-3(4H)-one[HMBOA];2,4- dihydroxy-2H-1,4-benzoxazin-3(4H)-one[DIBOA]; 2,4-dihydroxy-7-methoxy-2H-1,4-benzoxazin-3(4H)-one[DIMBOA]; and 2,4-dihydroxy-7,8-dimethoxy-2H-1,4-benzoxazin-3(4H)-one[DIM 2BOA]) found in maize (Zea mays L.) extracts. Derivatized samples were chromatographed on columns with liquid phases of 2% DC-11 and 3% OV-17 and detected by flame ionization. Internal standards were methyl palmitate and methyl stearate on DC-11 and methyl behenate on OV-17. Detector response was linear to at least 5 nanomoles for TMS2-HBOA and TMS2-DIBOA and to 19 nanomoles for TMS2-DIMBOA. Standard errors of 2% or less were obtained when four replicate samples were analyzed. For each of the 15 maize lines examined, the amount of DIMBOA determined by GLC was directly proportional to the amount of ferric chloride-reactive material determined colorimetrically.

2 Present address: Division of Plant Industry, CSIRO, P.O. Box 1600, Canberra City, A.C.T. 2601, Australia.

3 Present address: Departamento de Biología, Facultad de Ciencias, Universidad de Chile, Casilla 653, Santiago, Chile.

4 Also Plant Disease Resistance Research Unit, ARS, U.S. Department of Agriculture.

1 Research cooperative with the College of Agricultural and Life Sciences, University of Wisconsin, Madison, and the Science and Education Administration, Federal Research, United States Department of Agriculture.






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Copyright © 1979 by the American Society of Plant Biologists