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Plant Physiology 63:195-200 (1979)
© 1979 American Society of Plant Biologists

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Articles

Synthesis of a Possible Precursor of {alpha}-Amylase in Wheat Aleurone Cells 1

Thomas W. Okita2, Roberto Decaleya3 and Lawrence Rappaport

a Plant Growth Laboratory and Department of Vegetable Crops, University of California, Davis, California 95616

{alpha}-Amylase from wheat aleurone (Triticum aestivum) was synthesized in a S-150 wheat germ readout system using polysomes, and a messenger RNA-dependent reticulocyte lysate system using polyadenylic acid [poly(A)]-enriched RNA. The product was analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, precipitation by specific {lambda}-globulin for {alpha}-amylase, and proteolysis. Two immunoprecipitated products were synthesized from the readout system, the predominant species migrating coincidentally with authentic {alpha}-amylase on sodium dodecyl sulfate-polyacrylamide gels. A putative precursor, 1,500 daltons larger, was evident but was less abundant. The relationship between the two polypeptides was established by proteolytic analysis using Staphylococcus aureus V8 protease. At least nine fragments were generated and were identical in both species. The poly(A)-enriched RNA synthesized only the putative precursor in the reticulocyte lysate system. Attempts to process the precursor to the mature size of {alpha}-amylase failed. These findings are discussed in connection with the signal hypothesis (proposed for the transport of proteins across membranes) and the mode of secretion of {alpha}-amylase in aleurone cells.

2 Present address: Department of Biochemistry and Biophysics, University of California, Davis, California 95616.

3 Present address: Department of Bioquimicá, ETSI Agronomos, Ciudad Universitaria, Madrid, Spain.

1 Supported in part by the B.O.Z. Organization.






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ASPB Publications PLANT PHYSIOLOGY® THE PLANT CELL
Copyright © 1979 by the American Society of Plant Biologists