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Aspects of 8-[¹⁴C]Benzylaminopurine Metabolism in Phaseolus vulgaris¹

^a Institute of Pomology, University of Padova, 35100 Padova, Italy

Phaseolus vulgaris L. plants were supplied through the root with [8-¹⁴C]benzylaminopurine ([¹⁴C]BA). Collections of root, apex, and leaves were made 8 and 48 hours after labeling; ethanolic extracts of tissues were purified and subjected to thin layer chromatography on silica gel and/or cellulose powder.

From [¹⁴C]BA at least two different metabolites originate, the BA riboside and ribotide. The balance among [¹⁴C]BA, the riboside, and the ribotide changed in time. The [¹⁴C]BA riboside was detected as the only labeled compound in the xylem sap collected from the stem. The biological function of BA riboside and ribotide is discussed.