This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in Web of Science Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Web of Science (54) Citing Articles via Google Scholar Google Scholar Articles by Tully, R. E. Articles by Nelsen, C. E. Search for Related Content PubMed PubMed Citation Articles by Tully, R. E. Articles by Nelsen, C. E. Agricola Articles by Tully, R. E. Articles by Nelsen, C. E.

Articles

Proline Accumulation in Water-stressed Barley Leaves in Relation to Translocation and the Nitrogen Budget ¹

^a MSU-DOE Plant Research Laboratory, Michigan State University, East Lansing, Michigan 48824

Mobilization of N from leaves of barley (Hordeum vulgare L.) during water stress, and the role of proline as a mobilized species, were examined in plants at the three-leaf stage. The plants responded to water stress by withdrawing about 25% of the total reduced N from the leaf blades via phloem translocation. Most of this N loss was during the first 2 days while translocation of ¹⁴C-photosynthate out of the stressed blade still remained active. Free proline accumulation in the blade was initially slow, and became more rapid during the 2nd day of stress. Although a major free amino acid, proline accounted for only about 5% of the total N (soluble + insoluble) retained in severely stressed blades. When the translocation pathway in water-stressed leaves was interrupted just below the blade by a heat girdle, a cold jacket, or by blade excision, N loss from the blade was prevented and proline began to accumulate rapidly on 1st day of stress. Little free proline accumulated in the blades until after the ability to translocate was lost. Proline was, however, probably not a major species of N translocated during stress, because proline N accumulation in heat-girdled stressed leaves was five times slower than the rate of total N export from intact blades.

This article has been cited by other articles:

				S. Rubio, L. Whitehead, T. R. Larson, I. A. Graham, and P. L. Rodriguez The Coenzyme A Biosynthetic Enzyme Phosphopantetheine Adenylyltransferase Plays a Crucial Role in Plant Growth, Salt/Osmotic Stress Resistance, and Seed Lipid Storage Plant Physiology, September 1, 2008; 148(1): 546 - 556. [Abstract] [Full Text] [PDF]

				P. E. Verslues and R. E. Sharp Proline Accumulation in Maize (Zea mays L.) Primary Roots at Low Water Potentials. II. Metabolic Source of Increased Proline Deposition in the Elongation Zone Plant Physiology, April 1, 1999; 119(4): 1349 - 1360. [Abstract] [Full Text]