Plant Physiol. Illumina
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Plant Physiology 63:696-699 (1979)
© 1979 American Society of Plant Biologists

This Article
Right arrow Full Text (PDF)
Right arrow A correction has been published
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Bryant, S. D.
Right arrow Articles by Lane, F. E.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Bryant, S. D.
Right arrow Articles by Lane, F. E.
Agricola
Right arrow Articles by Bryant, S. D.
Right arrow Articles by Lane, F. E.
Articles

Indole-3-acetic Acid Oxidase from Peas

I. Occurrence and Distribution of Peroxidative and Nonperoxidative Forms 1

Stephen D. Bryant2 and Forrest E. Lane

a Department of Botany and Bacteriology, University of Arkansas, Fayetteville, Arkansas 72701

Tissues of etiolated pea seedlings variety Alaska were examined for the presence of peroxidative and nonperoxidative forms of indoleacetic acid (IAA) oxidase. Enzymes were extracted in a sequence involving acetone powder preparation from pea tissues, buffer extraction of the powder, ammonium sulfate precipitation, dialysis, lyophilization, and acrylamide gel electrophoresis. Electrophoretically separable proteins were assayed for IAA oxidase activity with the Salkowski test, and peroxidase activity was based on the color reaction with benzidine and H2O2. Each tissue examined contained several nonperoxidative IAA oxidases. No tissue contained more than three peroxidative IAA oxidases, whereas the plumule hooks (a tissue with a high IAA oxidase activity) contained no detectable peroxidases. The results indicate that nonperoxidative IAA oxidases might play a major role in the regulation of IAA content in pea seedlings.

2 Present address: TransAgra Corporation, 1355 Lynnfield Road, Memphis, Tennessee 38138.

1 Supported partially by an NDEA Title IV Fellowship to S. D. B.




This article has been cited by other articles:


Home page
 J Exp BotHome page
C. Yin, L. Gan, D. Ng, X. Zhou, and K. Xia
Decreased panicle-derived indole-3-acetic acid reduces gibberellin A1 level in the uppermost internode, causing panicle enclosure in male sterile rice Zhenshan 97A
J. Exp. Bot., July 1, 2007; 58(10): 2441 - 2449.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
ASPB Publications PLANT PHYSIOLOGY® THE PLANT CELL
Copyright © 1979 by the American Society of Plant Biologists