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Articles

Cysteinyl-tRNA Synthetase from Astragalus Species ¹

Alex Shrift^b

^a School of Environmental and Life Sciences, Murdoch University, Murdoch, 6153, Western Australia, Department of Biological Sciences, State University of New York, Binghamton, New York 13901

L-Cysteinyl-tRNA synthetases (EC 6.1.1.16) from four Astragalus species were partially purified. The substrate specificities of the cysteinyl-tRNA synthetase from three selenium accumulator species (A. crotalariae, A bisulcatus, and A. racemosus) were compared with those from two nonaccumulator species (A. lentigenosus and Phaseolus aureus). All species had similar K_m values for cysteine, selenocysteine, and -aminobutyric acid except A. bisulcatus which failed to use selenocysteine as a substrate and which had a K_m for cysteine four times greater than the K_m values for other species.