Plant Physiol. Drug Metab Dispos
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Plant Physiology 64:162-164 (1979)
© 1979 American Society of Plant Biologists

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Synchronization of Somatic Embryogenesis in a Carrot Cell Suspension Culture

Tatsuhito Fujimura and Atsushi Komamine

1 Department of Botany, Faculty of Science, University of Tokyo, Tokyo 113, Japan

Synchronization of somatic embryogenesis was achieved in a carrot (Daucus carota L. cv. "Kurodagosun") suspension culture by sieving the initial heterogeneous cell population, by density gradient centrifugation in Ficoll solutions, and by subsequent repeated centrifugations at a low speed (50g) for a short time (5 seconds), followed by transferring the cell clusters obtained, which were composed of 3 to 10 cells, to a medium containing zeatin (0.1 micromolar) but no auxin. The frequency of embryo formation reached more than 90%, and synchrony of the embryogenetic process was observed at least in the early stages of the process. The system established in the present work provides a useful system for biochemical research into the mechanisms of somatic embryogenesis.




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K. Takahata, M. Takeuchi, M. Fujita, J. Azuma, H. Kamada, and F. Sato
Isolation of Putative Glycoprotein Gene from Early Somatic Embryo of Carrot and its Possible Involvement in Somatic Embryo Development
Plant Cell Physiol., November 15, 2004; 45(11): 1658 - 1668.
[Abstract] [Full Text] [PDF]


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Copyright © 1979 by the American Society of Plant Biologists