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Plant Physiology 64:43-48 (1979)
© 1979 American Society of Plant Biologists

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Articles

Abscisic Acid Localization and Metabolism in Barley Aleurone Layers

William V. Dashek1, Bibhuti N. Singh and Daniel C. Walton2,3

a Department of Environmental and Forest Biology, State University of New York, College of Environmental Science and Forestry, Syracuse, New York 13210

Aleurone layers of Hordeum vulgare, cv. `Himalaya' took up [14C]-abscisic acid (ABA) when incubated for various times. Radioactivity accumulated with time in a low speed, DNA-containing pellet accounting for 1.6 to 2.3% of the radioactivity recovered in subcellular fractions at 18 hours. Thin layer chromatography of ethanolic or methanolic extracts of the cytosol, which contained greater than 95% of the radioactivity taken up by layers, revealed that labeled ABA was metabolized to phaseic acid (PA) and 4'-dihydrophaseic acid (DPA) and three polar metabolites Mx1, Mx2, and Mx3. ABA was not metabolized by endosperm, incubated under conditions used for layers, indicating that metabolism was tissue-specific. Layers metabolized [3H]DPA to Mx1 and Mx2. ABA, PA, and DPA-methyl ester and epi-DPA-methyl ester inhibited synthesis of {alpha}-amylase by layers incubated for either 37 or 48 hours. These layers converted the methyl DPA and epi-methyl-DPA esters to their respective acids. DPA did not inhibit Lactuca sativa germination or root and coleoptile elongation of germinating Hordeum vulgare seeds, or coleoptile elongation of germinating Zea mays seeds.

1 Present address: Department of Biology, West Virginia University, Morgantown, West Virginia 26506.

2 Work was supported by funds from the Herman Frasch Foundation and National Science Foundation Grant PCM 76-81196 to D. C. W.

3 To whom reprint requests should be sent.




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Q. Qi, P. A. Rose, G. D. Abrams, D. C. Taylor, S. R. Abrams, and A. J. Cutler
(+)-Abscisic Acid Metabolism, 3-Ketoacyl-Coenzyme A Synthase Gene Expression, and Very-Long-Chain Monounsaturated Fatty Acid Biosynthesis in Brassica napus Embryos
Plant Physiology, July 1, 1998; 117(3): 979 - 987.
[Abstract] [Full Text]


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Copyright © 1979 by the American Society of Plant Biologists