Plant Physiol.
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Plant Physiology 64:327-331 (1979)
© 1979 American Society of Plant Biologists

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Articles

Phytochrome Radioimmunoassay 1

Robert E. Hunt2 and Lee H. Pratt3

a Department of Biology, Vanderbilt University, Nashville, Tennessee 37235

A phytochrome radioimmunoassay with a detection limit of about 2 nanograms has been developed. The radioimmunoassay does not suffer from the potential drawbacks of the commonly used spectral assay and requires less than 1 microliter of crude extract from dark-grown plants for quantitation of phytochrome. Measurement of phytochrome in crude extracts by radioimmunoassay gives values about 25% greater than those obtained by spectral assay. The amount of phytochrome detected in crude extracts of light-grown oats by radioimmunoassay is approximately 1% of that detected in comparable extracts from dark-grown oats. General interference by crude plant extracts with radioimmunoassays was also observed and corrected for.

2 Present address: Section of Biochemistry, Cell and Molecular Biology, Cornell University, Ithaca, New York 14853.

3 Present address: Botany Department, The University of Georgia, Athens, Georgia 30602.

1 This research was supported by National Science Foundation Grants PCM75-14161, PCM75-19125, and PCM77-23584.






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Copyright © 1979 by the American Society of Plant Biologists