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Plant Physiology 64:332-336 (1979)
© 1979 American Society of Plant Biologists

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Articles

Phytochrome Immunoaffinity Purification 1

Robert E. Hunt2 and Lee H. Pratt3

a Department of Biology, Vanderbilt University, Nashville, Tennessee 37235

We have developed a phytochrome immunoaffinity purification procedure that yields undegraded oat (Avena sativa L., cv. Garry) phytochrome of greater than 98% purity within 2 hours when starting with a brushite-purified preparation. Immunoaffinity-purified phytochrome, except for its greater purity, is indistinguishable from conventionally purified phytochrome by gel exclusion chromatography, isoelectric focusing, and sodium dodecyl sulfate-polyacrylamide gel electrophoresis. We have also used the immunoaffinity technique to purify phytochrome from crude oat extracts, and from brushite-purified pea (Pisum sativum L., cv. Alaska) and rye (Secale cereale L., cv. Balbo) preparations.

2 Present address: Section of Biochemistry, Cell and Molecular Biology, Cornell University, Ithaca, New York 14853.

3 Present address: Botany Department, The University of Georgia, Athens, Georgia 30602.

1 This work was supported by National Science Foundation Grant PCM77-23584.






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ASPB Publications PLANT PHYSIOLOGY THE PLANT CELL
Copyright © 1979 by the American Society of Plant Biologists