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Enzymic Mechanism of Starch Breakdown in Germinating Rice Seeds

8. Immunohistochemical Localization of -Amylase

School of Agriculture, Nagoya University, Chikusa, Nagoya 464 Japan

Rabbit antiserum against -amylase isolated from germinating seeds of rice was produced, and its specific cross-reactivity with -amylase was confirmed by means of Ouchterlony double immunodiffusion and immunoelectrophoresis procedures. The cellular localization of -amylase was studied by indirect fluorescence microscopy of thin sectioned germinating rice seed specimens (1-day stage) which had been fixed and treated with purified rabbit anti--amylase immunoglobulin G followed by conjugation with fluorescein isothiocyanate-labeled goat antirabbit immunoglobulin G. It has been demonstrated that -amylase is uniformly associated with the periphery of starch granules in the starchy endosperm cells. The finding is discussed in relation to the general notion concerning the presence of the latent form of -amylase bound to protein bodies in cereal seeds.

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