Plant Physiol.
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Plant Physiology 64:668-670 (1979)
© 1979 American Society of Plant Biologists

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Thymidylate Synthase Activity from Chlamydomonas Cells and Cultured Tissues of Nicotiana, Pinus, and Daucus1

Vernon V. Vandiver, Jr.2 and Roger C. Fites

a Department of Botany, North Carolina State University, Raleigh, North Carolina 27650

Prior preparation of N5,N10-methylenetetrahydrofolate from DL-tetrahydrofolate and [14C]formaldehyde resulted in an improved assay for thymidylate synthase. Although preparations from tobacco seedlings and cotton root tips (0.25 centimeter) were inconsistent with respect to enzyme activity, extracts from actively growing cell cultures of Chlamydomonas, Nicotiana hybrid callus, Pinus callus, and Daucus proembryonic cells contained significant levels of thymidylate synthase (12.3 to 23.8 nanomoles of thymidylate formed per milligram of protein per hour).

2 Present address: Agricultural Research Center, University of Florida, Fort Lauderdale, Florida 33314.

1 Paper No. 5798 of the Journal Series of the North Carolina Agricultural Research Service, Raleigh, North Carolina.






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Copyright © 1979 by the American Society of Plant Biologists