Plant Physiol. Illumina
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Plant Physiology 64:671-673 (1979)
© 1979 American Society of Plant Biologists

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Articles

Comparison of Large Subunits of Type II DNA-dependent RNA Polymerases from Higher Plants 1

George H. Kidd2, Gerhard Link3 and Lawrence Bogorad

a The Biological Laboratories, Harvard University, Cambridge, Massachusetts 02138

Two-dimensional tryptic mapping of 125I-labeled polypeptides has been employed to compare the large subunits of type II DNA-dependent RNA polymerases from maize, parsley (Petroselinum sativum), and wheat. Maps of the 220 kilodalton (kd) and 140 kd subunits from wheat RNA polymerase II differ from those of the corresponding subunits from parsley enzyme II. The 180 kd subunits from maize and parsley type II enzymes also yield dissimilar tryptic maps. Thus, despite similarities in molecular mass, the large subunits of wheat, parsley, and maize type II RNA polymerases are unique to each individual plant species.

2 To whom correspondence should be addressed. Present address: Battelle-Columbus Laboratories, 505 King Avenue, Columbus, Ohio 43201. GHK was a predoctoral trainee of the National Institutes of Health on Multibiological Sciences Training Grant T01-GM-00036.

3 Supported by the Deutsche Forschungsgemeinschaft.

1 This work was supported in part by National Institute of General Medical Sciences Grant 20470 and in part by the Maria Moors Cabot Foundation of Harvard University.






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Copyright © 1979 by the American Society of Plant Biologists