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Plant Physiology 64:744-748 (1979)
© 1979 American Society of Plant Biologists

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On the Mechanism of Activation by Light of the NADP-dependent Malate Dehydrogenase in Spinach Chloroplasts

Renate Scheibe and Erwin Beck

Lehrstuhl für Pflanzenphysiologie, Universität Bayreuth, Am Birkengut, D-8580 Bayreuth, West Germany

With intact spinach (Spinacia oleracea L. cv. Vital R) chloroplasts, the activity of the NADP-dependent malate dehydrogenase after activation by light was 30 micromoles of malate formed per milligram of chlorophyll per hour; an identical rate of O2 evolution was obtained upon oxaloacetate reduction by the intact plastids. However, when the activity of NADP-dependent malate dehydrogenase was measured subsequently to maximal activation of the enzyme by dithiothreitol (DTT) an average rate of 113 micromoles per milligram of chlorophyll per hour was obtained. When membranes and stroma were separated after osmotic disruption of the chloroplasts, 28% of NADP-dependent malate dehydrogenase activity inducible by DTT was found with the membranes and 72% was found in the stromal fraction. The membrane-associated portion of the enzyme corresponds well with the activity achieved after activation by light. About 64% of an activator system was found to be associated also with the membrane fraction. Washing the membranes with buffer removed more activator than enzyme. However, both were removed almost completely by ethylenediaminetetraacetate. It was concluded that both a portion of the enzyme and the total activator system are associated with the chloroplast membranes in vivo and that the activator is more loosely bound than the enzyme. A model describing the partial activation of chloroplastic NADP-dependent malate dehydrogenase by light and the total activation by DTT is presented.




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M. Berkemeyer, R. Scheibe, and O. Ocheretina
A Novel, Non-redox-regulated NAD-dependent Malate Dehydrogenase from Chloroplasts of Arabidopsis thaliana L.
J. Biol. Chem., October 23, 1998; 273(43): 27927 - 27933.
[Abstract] [Full Text] [PDF]


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Copyright © 1979 by the American Society of Plant Biologists