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Plant Physiology 64:757-762 (1979)
© 1979 American Society of Plant Biologists

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Oxidative Phosphorylation in Pea Cotyledon Submitochondrial Particles 1

Charles Grubmeyer2, Dara Melanson, Ian Duncan and Mary Spencer3

a Department of Plant Science, University of Alberta, Edmonton, Alberta T6G 2N2 Canada

Mitochondria and submitochondrial particles (SMP) from pea cotyledons were shown to catalyze oxidative phosphorylation as measured by 32Pi uptake into phosphate esters. ATP synthesis was sensitive to the electron transport inhibitor KCN, the uncoupler carbonyl cyanide m-chlorophenylhydrazone, and the coupling factor inhibitor oligomycin. Experiments with the adenine nucleotide translocator inhibitor atractyloside indicated the SMP were inside-out. Mersalyl completely inhibited ATP synthesis by SMP, and a separate experiment indicated that mersalyl has a direct effect on the ATPase complex. The kinetics of ATP synthesis indicated a high affinity for phosphate (Km = 0.18 millimolar). ADP kinetics gave a biphasic curve with Km values of about 4.8 and 160 micromolar. O2 uptake and ATP synthesis had a pH maximum of 7.6 while the ratio of micromoles phosphate esterified to microatoms O2 taken up was highest at pH 7.2. Sodium chloride inhibited both ATP synthesis and O2 uptake but stimulated the ATPase reaction. The SMP also catalyzed a slow ATP-phosphate exchange reaction.

2 Present address: Department of Biochemistry, Public Health Research Institute of the City of New York, Inc., 455 First Avenue, New York, N. Y.

3 To whom reprint requests should be addressed.

1 We gratefully acknowledge grant A-1451 from the National Research Council of Canada in aid of this research, a Postgraduate Scholarship to CG and Postdoctoral Fellowship to DM.






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Copyright © 1979 by the American Society of Plant Biologists