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Euglena gracilis Chloroplast DNA Codes for Polyadenylated RNA ¹

Dennis E. Buetow^4,b

^a Department of Microbiology, University of Illinois, Urbana, Illinois 61801, Department of Physiology and Biophysics, University of Illinois

Polyadenylated RNA, isolated from total cellular RNA of photoautotrophically grown Euglena gracilis, comprised 2.1% of the total cellular RNA and contained 6.2% polyadenylic acid. Polyadenylated RNA, labeled in vitro with ¹²⁵I, hybridized at saturating levels to an average 7.7% of the chloroplast DNA. In the presence of excess chloroplast rRNA, hybridization of polyadenylated RNA was reduced, but was still observed at a level corresponding to 2.8% of the chloroplast DNA. Polyadenylic acid was not detected in mRNA prepared from chloroplast polyribosomes, indicating a level of less than 0.1% polyadenylic acid in mature chloroplast mRNA. Of the total RNA isolated from cytoplasmic polyribosomes, 2.0% contained polyadenylic acid. This latter polyadenylated RNA did not hybridize to chloroplast DNA.

³ Present address: Biochemical Development Division, Eli Lilly and Co., Indianapolis, Indiana 46206.

⁴ To whom reprint requests should be sent.

¹ This work was supported by National Science Foundation Grant GB 41928 and National Institutes of Health Grant GM 22431. A portion of these data has been presented previously (22).