This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Jung, D. W. Articles by Brierley, G. P. Search for Related Content PubMed PubMed Citation Articles by Jung, D. W. Articles by Brierley, G. P. Agricola Articles by Jung, D. W. Articles by Brierley, G. P.

Articles

Swelling and Contraction of Potato Mitochondria ¹

^a Department of Physiological Chemistry, Ohio State University, Columbus, Ohio 43210

Mitochondria isolated from potato tubers fail to undergo passive osmotic swelling when suspended in isotonic Na⁺ acetate or phosphate, in NaCl following addition of tripropyltin, or in Na⁺ nitrate following addition of an uncoupler. Swelling under each of these conditions in mitochondria from other sources has been attributed to the inward movement of Na⁺ on an endogenous Na⁺/H⁺ exchanger. Such a monovalent cation/H⁺ exchanger has also been implicated in respiration-dependent cation extrusion and contraction of swollen mitochondria. Potato mitochondria swollen in chloride and nitrate salts extrude ions and contract when respiration is initiated. The contraction reaction is slower and less efficient than that in beef heart mitochondria, but like the latter, is sensitive to uncouplers and stimulated by nigericin, butacaine, and Mg²⁺. These comparative studies suggest that a cation⁺/H⁺ exchanger is present in potato tuber mitochondria, but that it functions exclusively as a cation-extruding mechanism. They further suggest that cation⁺/H⁺ exchange activity is not identical in mitochondria from different sources and that these exchange components may have a directionality and regulatory features which differ with the metabolic needs of the source tissue.