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Articles

Ion Transport in Isolated Protoplasts from Tobacco Suspension Cells

III. Membrane Potential ¹

^a Department of Botany and Plant Sciences, University of California, Riverside, California 92521

The membrane electrical potential difference was measured in cultured cells and isolated protoplasts of tobacco (Nicotiana glutinosa L.) by inserting a microelectrode into cells held fast by a suction micropipette. The potential difference (± standard deviation) for unplasmolyzed tobacco cells was –52 ± 12 millivolts, for cells in 0.3 molar mannitol, –50 ± 11 millivolts; and for cells plasmolyzed in 0.7 molar mannitol, –49 ± 12 millivolts all inside negative. The potential difference for isolated protoplasts in 0.7 molar mannitol was –49 ± 16 millivolts, inside negative. In both cultured cells and protoplasts, the addition of 0.1 millimolar KCN caused a depolarization of the membrane potential. It was concluded that plasmolysis and enzymic release of the protoplast had no significant effect on the membrane potential of cultured tobacco cells.

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